Multidirectional approaches on autofermented chamomile ligulate flowers: Antioxidant, antimicrobial, cytotoxic and enzyme inhibitory effects

• Published in: South African journal of botany Vol. 120; pp. 112 - 118
• Main Authors: Cvetanović, A., Zeković, Z., Zengin, G., Mašković, P., Petronijević, M., Radojković, M.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.01.2019
• Subjects: Autofermentation; Biological activity; Chamomile; Enzyme-inhibitions; Natural agents; Natural agents; Chamomile; Autofermentation; Biological activity; Enzyme-inhibitions
• ISSN: 0254-6299; 1727-9321
• DOI: 10.1016/j.sajb.2018.01.003

In the frame of the present paper the enzymatic transformation of apigenin-glucosides into free aglycone was achieved by autofermentation of chamomile ligulate flowers (CLF). Antioxidant properties of the autofermented CLF (A-CLF) extract were evaluated by their radical scavenging activity against hydroxyl radicals and inhibition of lipid peroxidation. Obtained results showed that A-CLF extract in a concentration of 0.84mg/mL was able to inhibit 50% of hydroxyl radicals, while IC50 value in the case of inhibition of lipid peroxidation was 5.21mg/mL. Antimicrobial activity was done by measuring minimal inhibitory concentration (MIC) values for eight microbial strains. Obtained MIC values (9.75–156.25μg/mL) confirmed high antibacterial and antifungal activities of the extract. Cytotoxic activity was done by using three histological different cell lines: Hep2C; RD and L2OB. Obtained IC50 values for these cell lines were: 28.72; 17.31 and 10.92, respectively. Furthermore, in vitro investigation of the A-CLF ability to inhibit selected enzymes (α-amylase, α-glucosidase, tyrosinase) was done as well. Determined activities against α-amylase and α-glucosidase were 0.94 and 3.24mmolACAE/g, respectively. Further, measured activity against tyrosinase was 0.69mgKAEs/g indicating high enzyme-inhibitory activity of examined sample. Results demonstrated that A-CLF extracts showed considerable pharmacological activity. •Transformation of apigenin glycosides into apigenin by autofermentation of chamomile•Determination of antioxidant properties of extracts•Antimicrobial properties of extracts were examined using six indicator strains.•Using different cell lines cytotoxic activity of examined extracts was determined.•Enzyme-inhibitory activity was determined against amylase, glucosidase and tyrosinase.