Cell culture system versus adventitious root culture system in Asian and American ginseng: a collation
Panax ginseng and Panax quinquefolius of Panax genus are valuable as health foods as well as pharmaceuticals for the treatment of cancer, diabetes and ageing as these plants possess saponins. In the current study, Cell and adventitious root cultures of P. ginseng and P. quinquefolius were investigat...
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Published in | Plant cell, tissue and organ culture Vol. 132; no. 2; pp. 295 - 302 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.02.2018
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Panax ginseng
and
Panax quinquefolius
of
Panax
genus are valuable as health foods as well as pharmaceuticals for the treatment of cancer, diabetes and ageing as these plants possess saponins. In the current study, Cell and adventitious root cultures of
P. ginseng
and
P. quinquefolius
were investigated for the biomass, cell division, saponin content and ginsenosides profile from four lines namely
P. quinquefolius
(AM),
P. ginseng
mountain (Mt.) Baekdu line,
P. ginseng
Cheong-sol line (CS) and
P. ginseng
CBN line (CBN) with the objective of comparing cell and adventitious root systems to check their efficacy for the production of ginseng saponins. Additionally, genes related to ginsenoside biosynthesis were also analyzed concerning to cell and adventitious root lines. The results indicated that various cell lines were better in multiplication and growth compared to adventitious root lines. However, adventitious root lines showed higher accumulation of dry biomass (1.5–2 fold) than that of cell lines. CS adventitious root line showed higher saponin content and ginsenoside productivity (10.48 mg·g
−1
DW, 12.88 mg·L
−1
, respectively) than that of CS cell line (9.50 mg·g
−1
DW, 2.39 mg·L
−1
, respectively). Especially, Rd ginsenoside productivity of CS adventitious root line recorded fourfold higher than CS cell line. Genes which are related to ginsenoside biosynthesis such as
P. ginseng
squalene synthase (
PgSS2
),
P. ginseng
squalene epoxidase (
PgSE2
),
P. ginseng
protopanaxadial synthase (
PgPPDS
) and
P. ginseng
protopanaxatriol synthase (
PgPPTS
) were analyzed by real time quantitative polymerase chain reaction to support ginsenoside production. The adventitious root culture system described in this study is useful system for biomass and ginsenoside production. |
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ISSN: | 0167-6857 1573-5044 |
DOI: | 10.1007/s11240-017-1329-x |