Stage-dependent phosphoproteome remodeling of Parkinson's disease blood cells

• Published in: Neurobiology of disease Vol. 200; p. 106622
• Main Authors: Massacci, Giorgia, Venafra, Veronica, Zwiebel, Maximilian, Wahle, Maria, Cerroni, Rocco, Bissacco, Jacopo, Perfetto, Livia, Michienzi, Vito, Stefani, Alessandro, Mercuri, Nicola Biagio, Schirinzi, Tommaso, Sacco, Francesca
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2024; Elsevier
• Subjects: Biomarker; Parkinson's disease; Peripheral blood; Phosphoproteomic; Biomarker; Parkinson's disease; Phosphoproteomic; Peripheral blood
• ISSN: 0969-9961; 1095-953X; 1095-953X
• DOI: 10.1016/j.nbd.2024.106622

The complexity and heterogeneity of PD necessitate advanced diagnostic and prognostic tools to elucidate its molecular mechanisms accurately. In this study, we addressed this challenge by conducting a pilot phospho-proteomic analysis of peripheral blood mononuclear cells (PBMCs) from idiopathic PD patients at varying disease stages to delineate the functional alterations occurring in these cells throughout the disease course and identify key molecules and pathways contributing to PD progression. By integrating clinical data with phospho-proteomic profiles across various PD stages, we identify potential stage-specific molecular signatures indicative of disease progression. This integrative approach allows for the discernment of distinct disease states and enhances our understanding of PD heterogeneity. •PBMCs (phospho)proteomics reflects stage-specific signatures of PD.•Network-based approach reveals key signaling axes for PD course.•Machine learning-based strategy supports discovery of novel molecular targets.