Optimization of degradation of methylphosphonate — analogue of toxic pollutants with direct CP bond by Escherichia coli

Various strains of Escherichia coli were shown to be capable of utilizing methyl phosphonic acid (Pn) as a sole phosphorus source with resulting methane formation. The efficiency of this process depends on the age and concentration of culture cells. Aeration was an important factor regulating Pn deg...

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Bibliographic Details
Published inProcess biochemistry (1991) Vol. 36; no. 8; pp. 821 - 827
Main Authors Matys, S.V, Laurinavichius, K.S, Krupyanko, V.I, Nesmeyanova, M.A
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 2001
Subjects
Adaptation
Biodegradation
Escherichia coli
Organophosphonate
Biodegradation
Escherichia coli
Adaptation
Organophosphonate
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Summary:Various strains of Escherichia coli were shown to be capable of utilizing methyl phosphonic acid (Pn) as a sole phosphorus source with resulting methane formation. The efficiency of this process depends on the age and concentration of culture cells. Aeration was an important factor regulating Pn degradation: anaerobic conditions were more favourable for the process. For effective Pn degradation, cells need a long (72 h) period of adaptation to Pn. Adapted cells are able to utilize Pn with an order of efficiency higher than the initial cell culture. Adapted cells contain additional proteins, probably associated with Pn degradation, in different cell compartments and maintain this ability when placed onto fresh media even containing orthophosphate. Adapted cells are probably the best source of CP lyase for its isolation and study.
ISSN:1359-5113
1873-3298
DOI:10.1016/S0032-9592(00)00294-6