Tissue culture and metabolome investigation of a wild endangered medicinal plant using high definition mass spectrometry

• Published in: Plant cell, tissue and organ culture Vol. 134; no. 1; pp. 153 - 162
• Main Authors: Oliveira, J. P. S., Hakimi, O., Murgu, M., Koblitz, M. G. B., Ferreira, M. S. L., Cameron, L. C., Macedo, A. F.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.07.2018; Springer Nature B.V
• Subjects: Anthraquinones; Benzyladenine; Bioactive compounds; Biomedical and Life Sciences; Bioprospecting; Biotechnology; Conservation; Coumaric acid; Endangered plants; Gibberellic acid; Glucosinolates; Growth rate; Growth regulators; Herbal medicine; High definition; Indigenous plants; Inflammation; Investigations; Life Sciences; Mass spectrometry; Mass spectroscopy; Medicinal plants; Metabolites; Molecular chains; Multiplication; Organic acids; Original Article; Pharmacology; Phenolic compounds; Phenols; Plant Genetics and Genomics; Plant growth; Plant Pathology; Plant Physiology; Plant Sciences; Plantlets; Plants (botany); Propagation; Rooting; Rosmarinic acid; Steroids; Survival; Terpenes; Tissue culture; Wildlife conservation; Non-targeted; Metabolomics; UPLC–MS; In vitro; Growth regulators
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-018-1408-7

An increasing effort is dedicated to investigate the potential of native plants used in traditional medicine as a source of bioactive compounds for numerous industries. The bioprospection of the metabolome of medicinal and/or endangered plants has two important merits: confirming or revealing the biotechnological potential of that species, and assisting in its conservation. In addition, biotechnological techniques, such as tissue culture, are key strategies in conservation and multiplication of medicinal plants. This is the first in vitro development and non-targeted metabolome study by UPLC–QTOF–MS E of extracts from C. menthoides , an endangered medicinal plant. In vitro development investigation with a wide range of plant growth regulators resulted in maximum survival rate (81%) and the highest growth rate (1.74 cm ± 0.36) for plantlets cultured on Murashige and Skoog medium, supplemented with 1 µM gibberellic acid. Maximum rooting occurred on medium supplemented with 4.4 µM 6-benzyladenine, which also resulted in more leaves per plantlet (10.16 ± 1.7). We developed a protocol that can be used for the clonal propagation and ex situ conservation of this species. In terms of metabolome analysis, a total of 107 metabolites from several classes were detected and identified in its hydrophilic extract (HE), including organic acids and derivatives, glucosinolates, terpenes, phenolic compounds as well as other polar metabolites. The metabolites in HE with the greatest signal intensity included the isoquinoline alkaloid magnoflorine; the coumaric acid rosmarinic acid; the steroid-cardanolide convallatoxin; two anthraquinones including the poorly investigated ventinone A. Several molecules identified here carry potential pharmacological benefits such as anti-inflammatory and anticancer applications.