Human surfactant proteins A2 (SP-A2) and B (SP-B) genes as determinants of respiratory distress syndrome

• Published in: Indian pediatrics Vol. 52; no. 5; pp. 391 - 394
• Main Authors: Abuelhamed, Walaa A., Zeidan, Nancy, Shahin, Walaa A., Rizk, Hoda I., Rabie, Walaa A.
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 01.05.2015
• Subjects: Cross-Sectional Studies; Egypt - epidemiology; Female; Genetic Predisposition to Disease - genetics; Humans; Infant, Newborn; Maternal and Child Health; Medicine; Medicine & Public Health; Pediatric Surgery; Pediatrics; Polymorphism, Single Nucleotide - genetics; Pregnancy; Pulmonary Surfactant-Associated Protein A - genetics; Pulmonary Surfactant-Associated Protein B - genetics; Research Paper; Respiratory Distress Syndrome, Newborn - epidemiology; Respiratory Distress Syndrome, Newborn - genetics; Egypt; Surfactant protein; Neonate; Respiratory distress syndrome; Polymorphisms
• ISSN: 0019-6061; 0974-7559
• DOI: 10.1007/s13312-015-0643-9

Objective To study the relationship between SP-A2 and SP-B gene polymorphisms and respiratory distress syndrome in preterm neonates. Design Cross-sectional. Setting Neonatal intensive care unit and the Molecular Biology unit of the Chemical Pathology Department, Kasr Alainy hospital, Cairo University. Participants Sixty-five preterm infants with respiratory distress syndrome and 50 controls. The genomic DNA was isolated using DNA extraction kits. SYBR Greenbased real-time PCR was used to determine the variant genotypes of SP-A2 c.751 G<A and SP-B c.8714 G<C single nucleotide polymorphisms. Results Homozygosity of SP-A (OR 46, 95% CI 14–151) and SP-B (OR 5.2, 95% CI 2.3–11.4) alleles increased the risk of respiratory distress syndrome. The logistic regression model showed that genotypes SP-A2 (OR 164) and SP-B (OR 18) were directly related to the occurrence of respiratory distress syndrome, whereas gestational age (OR 0.57) and 5-minute Apgar score (OR 0.19) were inversely related to its occurrence. Conclusions There is a possible involvement of SP-A2 and SP-B genes polymorphisms in the genetic predisposition to respiratory distress syndrome.