Compatibility of DNA IQ™, QIAamp® DNA Investigator, and QIAsymphony® DNA Investigator® with various fingerprint treatments

• Published in: International journal of legal medicine Vol. 131; no. 2; pp. 293 - 301
• Main Authors: Lin, Sze-wah, Ip, Stephen C. Y., Lam, Tze-tsun, Tan, Tung-fai, Yeung, Wai-lung, Tam, Wai-ming
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.03.2017
• Subjects: Cyanoacrylates; Dermatoglyphics; DNA - isolation & purification; DNA Fingerprinting - instrumentation; Forensic Medicine; Humans; Indans; Maleates; Medical Law; Medicine; Medicine & Public Health; Microsatellite Repeats; Ninhydrin; Original Article; Polymerase Chain Reaction; Powders; Specimen Handling - instrumentation; Volatilization; Aluminum powder; Fingerprint; Magnetic powder; Physical Developer; Forensic DNA typing; Super Glue
• ISSN: 0937-9827; 1437-1596
• DOI: 10.1007/s00414-016-1447-8

Latent fingerprint and touch DNA are the two most important contact evidence for individualization in forensic science which provide complementary information that can lead to direct and unequivocal identification of the culprit. In order to retrieve useful information from both fingerprints and DNA, which are usually mingled together, one strategy is to perform fingerprint examination prior to DNA analysis since common DNA sampling technique such as swabbing could disturb or even destroy fingerprint details. Here, we describe the compatibility of three automatic DNA extraction systems, namely, DNA IQ™, QIAamp ® DNA Investigator, and QIAsymphony ® DNA Investigator ® , with respective to the effects of various fingerprint detection techniques. Our results demonstrate that Super Glue fingerprint treatment followed by DNA IQ™ extraction shows better effectiveness in DNA profiling. Aluminum powder dusting offers the least interference to the three DNA extraction systems above. Magnetic powder dusting, on the other hand, strongly impedes DNA recovery. Physical Developer is the most intrusive, which yields profiles with poor quality, including lower peak heights, poor peak height ratios, and poor intra-color balance. In terms of the choice of extraction method, DNA IQ™ system is recommended for sampling after fingerprint treatments, but not the two DNA Investigator systems.