Occurrence and genomic characterization of antimicrobial-resistant and potential pathogenic Escherichia coli from Italian artisanal food productions of animal origin

can harbor a broad repertoire of virulence and antimicrobial resistance (AMR) genes, which can be exchanged across the human gastrointestinal microflora, thus posing a public health risk. In this study, 6 batches of artisanal soft cheese and a 6-month ripened fermented dried sausage were investigate...

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Published inItalian journal of food safety Vol. 13; no. 2; p. 12205
Main Authors Crippa, Cecilia, De Cesare, Alessandra, Lucchi, Alex, Parisi, Antonio, Manfreda, Gerardo, Pasquali, Frédérique
Format Journal Article
LanguageEnglish
Published Italy PAGEPress Publications, Pavia, Italy 15.05.2024
PAGEPress Publications
Subjects
antimicrobial resistance
Artisanal food chain
Escherichia coli
plasmids
virulence
plasmids
virulence
antimicrobial resistance
Escherichia coli
artisanal food chain
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Summary:can harbor a broad repertoire of virulence and antimicrobial resistance (AMR) genes, which can be exchanged across the human gastrointestinal microflora, thus posing a public health risk. In this study, 6 batches of artisanal soft cheese and a 6-month ripened fermented dried sausage were investigated to assess the occurrence, phylogeny, and genomic traits (AMR, virulence, and mobilome) of . 30 and 3 strains isolated from salami and cheese food chains, respectively, were confirmed as by whole genome sequencing. The accumulation of single nucleotide polymorphism differences within small clusters of strains encompassing batches or processing stages, combined with high serotype and phylogroup diversity, suggested the occurrence of different contamination phenomena among the facilities. A total of 8 isolates harbored plasmid-mediated resistance genes, including one cheese strain that carried an IncQ1 plasmid carrying AMR determinants to macrolides [ (B)], sulfonamides ( , ), trimethoprim , and aminoglycosides [ (3")-Ib and (6)-Id]. A pool of virulence-associated genes in the class of adhesion, colonization, iron uptake, and toxins, putative -positive iron uptake systems , , or (8 salami and 2 cheese), plasmid-encoded hemolysin operon ABCD (one salami), and potential atypical enteropathogenic (3 salami environment) were reported. Overall, our findings underscore the importance of routine surveillance of in the artisanal food chain to prevent the dissemination of AMR and virulence.
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Conference presentation: this paper was presented at the XXXII National Conference of the Italian Association of Veterinary Food Hygienists (AIVI), Maierato-Vibo Valentia, September 13-14-15, 2023.
Availability of data and materials: data and materials are available from the corresponding author upon request.
Publisher's note: all claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Contributions: FP, GM, ADC, conceived and planned the study; CC, AL, carried out the laboratory experiments, including samples collection, strain’s isolation and phenotypic analyses as well as DNA extraction; AP, performed library preparation and DNA sequencing; CC, implemented the post-sequencing bioinformatic analyses and drafted the manuscript. All authors have supported to results interpretation and have commented and approved the final version as submitted.
Conflict of interest: the authors declare no potential conflict of interest.
Ethics approval and consent to participate: not applicable.
ISSN:2239-7132
2239-7132
DOI:10.4081/ijfs.2024.12205