Statistical optimization for enhanced bacoside A production in plant cell cultures of Bacopa monnieri

• Published in: Plant cell, tissue and organ culture Vol. 133; no. 2; pp. 203 - 214
• Main Authors: Leonard, J., Seth, Bishwanath, Sahu, Binod B., Singh, V. R., Patra, Nivedita
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.05.2018; Springer Nature B.V
• Subjects: Acetic acid; Alternative technology; Ammonium; Bacopa monnieri; Bioactive compounds; Biomass; Biomedical and Life Sciences; Cell culture; Cholinesterase; Computer simulation; Docking; Endangered species; Energy value; Inoculum; Life Sciences; Lymphocytes B; Naphthalene; Optimization; Original Article; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Potassium phosphate; Potassium phosphates; Sucrose; Sugar; Suspension culture; Bacoside A; Central composite design; Benzylaminopurine; Naphthalene acetic acid
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-017-1373-6

The bioactive compound, bacoside A, has immense importance for the treatment of memory disorders and Alzheimer’s disease. Due to the growing commercial interest in the herb, Bacopa monnieri , it has been listed as highly endangered species. The present study was aimed at enhancing the production of bacoside A using an alternative technology of plant cell suspension culture. Initial experiments of docking simulations using bacoside A showed good inhibition of acetyl cholinesterase (binding energy value of − 20 kcal/mol), when comparison was made with other phytocompounds and the synthetic drug for Alzheimer’s disease. In vitro experiments established that B. monnieri cell suspension culture can be developed in Murashige and Skoog medium containing containing 0.1 mg/L benzylaminopurine and 0.5 mg/L naphthalene acetic acid. Plackett–Burman studies predicted that the most effective factors for maximum biomass production were inoculum size (t-value of 4.87), sucrose concentration (t-value of 0.25) and KH 2 PO 4 concentration (t-value of 0.007). The nitrate to ammonium ratio (t-value of − 0.42) did not have significant effect on the cell suspension biomass. The optimum concentration of the crucial variables obtained from a central composite design were—inoculum size of 2 g/L, sucrose concentration of 30 g/L and KH 2 PO 4 concentration of 1.24 mM in one-sixth strength MS medium. The best model for optimum production of biomass and bacoside A was experimentally verified and the correlation between the predicted and actual values was found to be 99% for biomass and 94% for bacoside A production. The experimental results have been discussed in the present work.