Cocaine Abuse in Humans Is Not Associated with Increased Microglial Activation: An 18-kDa Translocator Protein Positron Emission Tomography Imaging Study with [11C]PBR28

• Published in: The Journal of neuroscience Vol. 34; no. 30; pp. 9945 - 9950
• Main Authors: Narendran, R., Lopresti, B. J., Mason, N. S., Deuitch, L., Paris, J., Himes, M. L., Kodavali, C. V., Nimgaonkar, V. L.
• Format: Journal Article
• Language: English
• Published: United States Society for Neuroscience 23.07.2014
• Subjects: Acetamides - metabolism; Adult; Brief Communications; Carbon Radioisotopes; Cocaine-Related Disorders - diagnosis; Cocaine-Related Disorders - metabolism; Cocaine-Related Disorders - pathology; Female; Humans; Male; Microglia - metabolism; Middle Aged; Positron-Emission Tomography - methods; Protein Binding - physiology; Pyridines - metabolism; Receptors, GABA - metabolism; TSPO; [11C]PBR28; cocaine dependence; inflammation; PET
• ISSN: 0270-6474; 1529-2401
• DOI: 10.1523/JNEUROSCI.0928-14.2014

Basic science investigations have consistently shown that repeated exposure to psychostimulant drugs, such as cocaine, activate the immune response and lead to inflammatory changes in the brain. No previous in vivo studies have confirmed this observation in chronic cocaine-abusing humans. To test this hypothesis, we used positron emission tomography imaging to measure the binding of [(11)C]PBR28 to the 18 kDa translocator protein (TSPO), a marker for microglial activation in a group of 15 recently abstinent cocaine abusers and 17 matched healthy controls. [(11)C]PBR28 volumes of distribution expressed relative to total plasma ligand concentration (VT) were measured in subjects with kinetic analysis using the arterial input function. Subjects were also genotyped for the TSPO alanine147 threonine (Ala147Thr, rs6971) polymorphism that has been shown to influence the in vivo binding of PBR28 to TSPO. Consistent with previous reports, the TSPO Ala147Thr genotype predicted the in vivo binding of [(11)C]PBR28. No significant differences in [(11)C]PBR28 VT were observed in the cortical and subcortical regions in cocaine abusers compared with healthy controls. The results of this in vivo study do not support increased TSPO expression and, by extension, microglial activation in chronic cocaine-abusing humans. Further research with more direct markers of microglial activation is necessary to conclusively rule out neuroinflammation in cocaine dependence.