Identification and characterization of brt1, a gene down-regulated during B-regulated development in Schizophyllum commune

• Published in: Current genetics Vol. 35; no. 5; pp. 551 - 556
• Main Authors: Lengeler, K.B, Kothe, E
• Format: Journal Article
• Language: English
• Published: United States 01.06.1999
• Subjects: amino acid sequences; Base Sequence; Blotting, Northern; brt1 gene; Chromosome Mapping; Cloning, Molecular; Crosses, Genetic; Deoxyribonuclease BamHI - genetics; Down-Regulation; Fungal Proteins - genetics; Fungal Proteins - metabolism; genbank/af118109; Gene Deletion; gene expression; Gene Expression Regulation, Fungal; genes; Genetic Markers; genetic regulation; Genetic Techniques; In Situ Hybridization - methods; mating behavior; mating type genes; messenger RNA; Molecular Sequence Data; nucleotide sequences; promoter regions; proteins; Reproduction - genetics; RNA, Fungal; Schizophyllum - genetics; Schizophyllum - growth & development; Schizophyllum commune; Sequence Homology, Amino Acid; sexual reproduction; Transcription, Genetic
• ISSN: 0172-8083; 1432-0983
• DOI: 10.1007/s002940050452

To identify genes regulated by the B mating-type genes a differential RNA display was established for the homobasidiomycete Schizophyllum commune and a gene, brt1, was identified. The mRNA concerned is highly abundant in monokaryotic mycelia and is down-regulated in matings in which either the B genes alone or else both the A and B genes are different between the mates. This places the gene brt1 under the control of the pheromone response system of S. commune encoded by the B mating-type loci. Sequence analysis revealed similarity to a novel protein family, two members of which have been shown to inhibit translational re-initiation.