Association of IL-10 Gene Polymorphism (−819C > T, −592C > A and −1082G > A) with Preterm Birth

• Published in: Indian journal of pediatrics Vol. 85; no. 2; pp. 93 - 101
• Main Authors: Pandey, Monika, Awasthi, Shally, Singh, Urmila, Mahdi, Abbas Ali
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 01.02.2018
• Subjects: Adolescent; Adult; Female; Genetic Association Studies; Gynecology; Haplotypes; Humans; Interleukin-10 - genetics; Linkage Disequilibrium - genetics; Medicine; Medicine & Public Health; Original Article; Pediatrics; Polymorphism, Single Nucleotide - genetics; Pregnancy; Premature Birth - genetics; Young Adult; Preterm birth; Cytokines; Interleukin; Inflammation; Gene expression; Polymorphisms; Haplotype
• ISSN: 0019-5456; 0973-7693; 0973-7693
• DOI: 10.1007/s12098-017-2496-9

Objective To analyze the association of IL-10 gene and its polymorphisms with preterm birth (PTB). Methods Five hundred and fifty nine women with term birth and 559 with preterm birth were recruited from Lucknow, India. Genetic association analysis was conducted between cases and controls. Subjects recruited as cases were women (aged between 18–40 y) with singleton delivery before 37 wk of gestation and controls were with delivery after or on 37 wk. The genotyping was performed for rs1800871, rs1800872 and rs1800896 for assessing the allelic distribution, haplotypic association and linkage disequilibrium analysis. IL-10mRNA levels were evaluated by real time quantitative polymerase chain reaction (PCR) method. Results The risk of PTB was found significant in women carrying IL-10 (−1082) GA genotype [OR=1.72(1.7–2.5), p =0.006]. The haplotypic analysis of studied polymorphisms for rs1800871, rs1800872 and rs1800896 depicted the association of ATA ( p =0.02) and ATC ( p =0.01) haplotypes with PTB. The IL-10 mRNA levels were significantly lower in cases ( p =0.05). Conclusions IL-10 marks a protective impact in the inflammatory pathway of PTB.