Optimization of extraction techniques and quantification of swertiamarin and mangiferin by using RP-UFLC method from eleven Swertia species

Present work investigated an efficient method for extraction of swertiamarin and mangiferin from different species of genus Swertia. Various extraction methods like static extraction (SE), continuous shaking extraction (CSE), and ultrasonic extraction (USE) were evaluated for increasing recovery per...

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Bibliographic Details
Published inSouth African journal of botany Vol. 108; pp. 81 - 89
Main Authors Kshirsagar, P.R., Gaikwad, N.B., Pai, S.R., Bapat, V.A.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.01.2017
Subjects
Extraction
Mangiferin
Optimization
RP-UFLC
Swertia
Swertiamarin
SAN
Swertia
SAP
SPA
SLA
Swertiamarin (CID:442435)
SNE
Optimization
SE
RP-UFLC
CSE
Mangiferin (CID:5281647)
SDE
SDI
Extraction
SBI
LOD
RSD
USE
Swertiamarin
SMI
LOQ
SCH
SCO
Mangiferin
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Summary:Present work investigated an efficient method for extraction of swertiamarin and mangiferin from different species of genus Swertia. Various extraction methods like static extraction (SE), continuous shaking extraction (CSE), and ultrasonic extraction (USE) were evaluated for increasing recovery percentage of swertiamarin and mangiferin. The quantification was done using reversed phase-ultra flow liquid chromatographic (RP-UFLC) method at 238nm (swertiamarin) and 257nm (mangiferin) wavelength. The results revealed that the percentage extraction of swertiamarin and mangiferin from different species of Swertia by SE was more proficient. Three time intervals were optimized by SE and it was observed that the 24h extraction gave the maximum recovery in S. chirayita of swertiamarin (256.98mg/g) and mangiferin (155.76±7.78mg/g). S. minor remarkably best match for S. chirayita as per phytochemical fingerprint using swertiamarin and mangiferin is concerned, suggesting an alternative for chirayita. •Optimization of extraction techniques of swertiamarin and mangiferin by RP-UFLC method from eleven Swertia species•SE, CSE, and USE techniques were evaluated for percent recovery of swertiamarin and mangiferin•SE technique was the most reliable and reproducible method towards highest recovery of swertiamarin and mangiferin•S. minor remarkably best match for S. chirayita, suggesting an alternative for chirayita
ISSN:0254-6299
1727-9321
DOI:10.1016/j.sajb.2016.09.016