Identification of dairy Propionibacterium species by rRNA gene restriction patterns

• Published in: Research in microbiology Vol. 145; no. 9; pp. 667 - 676
• Main Authors: de Carvalho, A.F., Gautier, M., Grimont, F.
• Format: Journal Article
• Language: English
• Published: Paris Elsevier Masson SAS 1994; Elsevier
• Subjects: Bacteriology; Biological and medical sciences; DNA CLEAVAGE; FRAGMENTACION DE LA ADN; FRAGMENTATION DE L'ADN; Fundamental and applied biological sciences. Psychology; GENETICA MOLECULAR; Genetics; GENETIQUE MOLECULAIRE; IDENTIFICACION; IDENTIFICATION; Identification, Subspecies, Lactic acid bacteria; Microbiology; MILK PRODUCTS; MOLECULAR GENETICS; PRODUCTOS LACTEOS; PRODUIT LAITIER; PROPIONIBACTERIUM; Propionibacterium acidipropionici; Propionibacterium freudenreichii; Propionibacterium jensenii; Propionibacterium, Ribotyping, Taxonomy; SOUS ESPECE; SUBESPECIES; SUBSPECIES; TAXONOMIA; TAXONOMIE; TAXONOMY; Propionibacterium, Ribotyping, Taxonomy; Identification, Subspecies, Lactic acid bacteria; Lactic acid bacteria; Propionibacterium; Typing; Genetic marker; Taxonomy; Ribosomal DNA; Chemotaxonomy; Genotype; Identification; Species specificity; Method; Restriction fragment length polymorphism; Propionibacteriaceae; Bacteria; Actinomycetes
• ISSN: 0923-2508; 1769-7123
• DOI: 10.1016/0923-2508(94)90038-8

A total of 78 strains of dairy propionibacteria, 4 reference strains of Propionibacterium and 8 related bacteria were characterized by ribosomal ribonucleic acid (rRNA) gene restriction patterns (ribotyping). The patterns were obtained after cleavage of total DNA with either BamHl or Clal restriction endonucleases and hybridization of fragments with acetylaminofluorene-labelled 16+23S rRNA from Escherichia coli. The four different species of dairy propionibacteria, P. freudenreichii, P. jensenii, P. thoenii and P. acidipropionici, gave different restriction patterns with species-specific fragments. Moreover, ribotyping allowed the differentiation of P. freudenreichii subsp. Freudenreichii from P. freudenreichii subsp. shermanii. The patterns of dairy propionibacteria were different from those of closely related bacteria and other bacteria used in the dairy industry.