Effect of oral administration of tri-o-cresyl phosphate on in vitro phosphorylation of membrane and cytosolic proteins from chicken brain

The effects of a single oral dose of 750 mg/kg tri-o-cresyl phosphate (TOCP) on the endogenous phosphorylation of specific brain proteins were assessed in male adult chickens following the development of delayed neurotoxicity. Phosphorylation of crude synaptosomal (P2) membrane and synaptosomal cyto...

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Bibliographic Details
Published inJournal of neurochemistry Vol. 41; no. 3; p. 897
Main Authors Patton, S E, O'Callaghan, J P, Miller, D B, Abou-Donia, M B
Format Journal Article
LanguageEnglish
Published England 01.09.1983
Subjects
Administration, Oral
Animals
Brain - metabolism
Chickens
Cresols - administration & dosage
Cytosol - metabolism
Electrophoresis, Polyacrylamide Gel
Male
Membrane Proteins - metabolism
Nerve Tissue Proteins - metabolism
Phosphorylation
Tritolyl Phosphates - administration & dosage
Tritolyl Phosphates - pharmacology
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Summary:The effects of a single oral dose of 750 mg/kg tri-o-cresyl phosphate (TOCP) on the endogenous phosphorylation of specific brain proteins were assessed in male adult chickens following the development of delayed neurotoxicity. Phosphorylation of crude synaptosomal (P2) membrane and synaptosomal cytosolic proteins was assayed in vitro by using [gamma-32P]ATP as phosphate donor. Following resolution of brain proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis, specific protein phosphorylation was detected by autoradiography and quantified by microdensitometry. TOCP administration enhanced the phosphorylation of both cytosolic (Mr 65,000 and 55,000) and membrane (20,000) proteins by as much as 146% and 200%, respectively.
ISSN:0022-3042
DOI:10.1111/j.1471-4159.1983.tb04826.x