Effect of extracellular matrix on two cell lines established from Mongolian gerbil's (Meriones unguiculatus) malignant melanoma

We investigated the effect of extracellular matrix (ECM) on the adhesion, morphology and proliferation of two Mongolian gerbil's melanoma cell lines (MGM-S, MGM-A) which differed in malignancy. Fibronectin matrix was detected on the cell surface of MGM-S by immunofluorescence techniques. MGM-A...

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Bibliographic Details
Published inJournal of Veterinary Medical Science Vol. 57; no. 4; pp. 635 - 640
Main Authors Hashimoto, O. (Tokyo Univ. (Japan). Faculty of Agriculture), Ogawa, K, Kurohmaru, M, Hayashi, Y
Format Journal Article
LanguageEnglish
Published Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.08.1995
Japan Science and Technology Agency
Subjects
ADHESIVIDAD
ADHESIVITE
Animals
Blotting, Western
Cell Adhesion
CELL CULTURE
Cell Division
Cell Line
Chromosome Aberrations
Chromosome Disorders
CRECIMIENTO
CROISSANCE
CULTIVO DE CELULAS
CULTURE DE CELLULE
CULTURE TECHNIQUES
Culture Techniques - methods
Extracellular Matrix - physiology
Female
Fibronectins - analysis
Gerbillinae
GLICOPROTEINAS
GLYCOPROTEINE
GLYCOPROTEINS
GROWTH
Kinetics
laminin
Male
malignancy
MELANOMA
Melanoma - genetics
Melanoma - pathology
Melanoma - veterinary
melanoma cell line
MELANOME
Mongolian gerbil
Neoplasm Transplantation
Ploidies
Rodent Diseases
RODENTIA
STICKINESS
TECHNIQUE DE CULTURE
TECNICAS DE CULTIVO
tibronectin
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Summary:We investigated the effect of extracellular matrix (ECM) on the adhesion, morphology and proliferation of two Mongolian gerbil's melanoma cell lines (MGM-S, MGM-A) which differed in malignancy. Fibronectin matrix was detected on the cell surface of MGM-S by immunofluorescence techniques. MGM-A cells, showing a multipolar shape, were widely spread over the dishes coated with fibronectin, laminin or type IV collagen. The adhesion rate of MGM-A cells to ECM components was higher compared to that in controls. Attachment to laminin was conspicuously promoted. The effect of laminin on MGM-A cell growth was also the highest among the substrates examined. On the other hand, these ECM components did not affect MGM-S cells. These results suggest that laminin and fibronectin may participate in the malignancy of MGM cell lines.
Bibliography:L74
9607635
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ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.57.635