In vitro observation of the molecular interaction between NodD and its inducer naringenin as monitored by fluorescence resonance energy transfer
At initial stages in the Rhizobium legume symbiosis, most Modulation genes are controlled by NodD protein and plant inducers. Some genetic studies and other reports have suggested that NodD may be activated by its direct interaction with plant inducers. However, there has been no molecular evidence...
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Published in | Acta biochimica et biophysica Sinica Vol. 40; no. 9; pp. 783 - 789 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Publishing Ltd
01.09.2008
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Subjects | |
Online Access | Get full text |
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Summary: | At initial stages in the Rhizobium legume symbiosis, most Modulation genes are controlled by NodD protein and plant inducers. Some genetic studies and other reports have suggested that NodD may be activated by its direct interaction with plant inducers. However, there has been no molecular evidence of such an inducing interaction. In this paper, we used fluorescence resonance energy transfer technique to see whether such an interaction exists between NodD and its activator, naringenin, in vitro. The tetracysteine motif (Cys‐Cys‐Pro‐Gly‐Cys‐Cys) was genetically inserted into NodD to label NodD with 4',5'‐bis(1,3,2‐dithioarsolan‐2‐yl) fluorescein (FlAsH). Naringenin was labeled with fluorescein by chemical linking. In the fluorescence resonance energy transfer experiments in vitro, the fluorescence intensity of one acceptor, NodD(90R6)‐FlAsH, increased by 13%. This suggests that NodD may directly interact with inducer naringenin in vitro and that the reaction centre is likely near hinge region 1 of NodD. |
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Bibliography: | The work was supported by grants from the National Basic Research Program of China (No. G2002CB713808) and the PanDeng Plan of China ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1672-9145 1745-7270 |
DOI: | 10.1111/j.1745-7270.2008.00462.x |