Kinetic analysis of Qa-1-specific cloned cytotoxic T lymphocytes: lytic parameters and evaluation of cellular inhibition

• Published in: Scandinavian journal of immunology Vol. 25; no. 6; p. 541
• Main Authors: LeFever, A V, Truitt, R L
• Format: Journal Article
• Language: English
• Published: England 01.06.1987
• Subjects: Animals; Antigens, Surface - immunology; Antigens, Surface - metabolism; Clone Cells; Cross Reactions; Epitopes; Histocompatibility Antigens Class I; Kinetics; Mice; Mice, Inbred Strains; T-Lymphocytes, Cytotoxic - metabolism
• ISSN: 0300-9475; 1365-3083
• DOI: 10.1111/j.1365-3083.1987.tb01080.x

A kinetic analysis of lysis assay was used to compare cytolysis of target cells expressing different allelic Qa-1 determinants by a Qa-1b-specific cytotoxic T-lymphocyte clone (CTL). Although this clone specifically recognized the Qa-1b determinant it also recognized the Qa-1c and Qa-1d determinants to a lesser extent. The maximum rate of lysis against Qa-1b targets was 10-fold faster than against Qa-1c or -1d targets. The affinity of the CTL clone for the Qa-1b and -1c target cells was the same, but significantly less for Qa-1d targets. The pattern of inhibition observed for target and inhibitor cells in these experiments was competitive. These studies demonstrated a greater similarity between the Qa-1b and -1c determinants, compared with Qa-1d. Kinetic analysis of lytic reactions allowed for quantitative evaluation of the similarities and differences between various target cell populations to an extent not possible using conventional cell-mediated assays.