Far-red stimulated long-lived luminescence from barley protoplasts

• Published in: Plant science (Limerick) Vol. 55; no. 1; pp. 1 - 7
• Main Authors: Nakamoto, Hitoshi, Sundblad, Lars-Göran, Gardeström, Per, Sundbom, Erik
• Format: Journal Article
• Language: English
• Published: Elsevier Ireland Ltd 1988
• Subjects: far-red stimulated luminescence; Hordeum vulgare; protoplasts; far-red stimulated luminescence; PS II; BSA; Hordeum vulgare; Hepes; DCMU; PS I; PVP40; FCCP; protoplasts
• ISSN: 0168-9452; 1873-2259; 1873-2259
• DOI: 10.1016/0168-9452(88)90035-0

The far-red stimulated luminescence decay kinetics, which in intact leaves from higher plants shows a relative maximum 30–90 s after excitation was studied on barley ( Hordeum vulgare) protoplasts. When protoplasts were preilluminated with white light prior to far-red excitation, the luminescence maximum was consistently observed. The effect of the inhibitors 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), hydroxylamine, antimycin A and glycoladehyde and the uncoupler carbonylcyanide-p- trifluoromethoxyphenylhydrazone (FCCP) showed that: (1) The luminsecence expressed as a maximum is emitted from PS II, although it is stimulated through PS I. (2) The maximum is dependent on: reverse electron flow utilizing parts of both the cyclic, and the linear electron transport pathways; a transthylakoid ΔpH. (3) Operation of a complete Calvin cycle is not necessary for the maximum to appear. We conclude that protoplasts are a suitable experimental system for further work on the underlying mechanism behind the far-red stimulated relative maximum in the minute range decay of luminescence.