The use of two-dimension electrophoresis to identify serum biomarkers from patients with dengue haemorrhagic fever

• Published in: Transactions of the Royal Society of Tropical Medicine and Hygiene Vol. 103; no. 4; pp. 413 - 419
• Main Authors: Thayan, Ravindran, Huat, Tan Lian, See, Lucy Lum Chai, Tan, Christina Phoay Lay, Khairullah, Nor Shahidah, Yusof, Rohana, Devi, Shamala
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.04.2009; Royal Society of Tropical Medicine and Hygiene; Oxford University Press
• Subjects: Adolescent; Adult; alpha 1-Antitrypsin - blood; alpha 1-Antitrypsin - immunology; Arboviral haemorrhagic fevers; Arboviroses; Biological and medical sciences; Biomarkers - blood; Dengue fever; Dengue fevers; Dengue haemorrhagic fever; Dengue Virus - immunology; Disease indicators; Electrophoresis, Gel, Two-Dimensional; Enzyme-Linked Immunosorbent Assay - methods; Female; General aspects; Human viral diseases; Humans; Immunoglobulin M - blood; Infectious diseases; Male; Medical sciences; NS1 protein; Protein marker; RNA, Viral - blood; Severe Dengue - diagnosis; Severe Dengue - immunology; Tropical viral diseases; Viral diseases; Viral Nonstructural Proteins - blood; Viral Nonstructural Proteins - immunology; Young Adult; α1-antitrypsin; α1-antitrypsin; Dengue haemorrhagic fever; Disease indicators; Dengue fever; NS1 protein; Protein marker; Human; Hemorrhagic fever; Dengue hemorrhagic fever; Dengue; Biological marker; Indicator; Protein; α1-Antitrypsin; Infection; Viral disease; Arbovirus disease; Electrophoresis; Tropical medicine; Serum
• ISSN: 0035-9203; 1878-3503
• DOI: 10.1016/j.trstmh.2008.12.018

Dengue infection is a major public health problem affecting millions of people living in tropical countries. With no suitable vaccines and specific antiviral drugs, treatment for dengue is usually symptomatic and supportive. Early diagnosis and recognition of severe disease is therefore crucial for better management of the patient. Two-dimension electrophoresis was used to identify disease-associated proteins that can be used for diagnosis and as drug targets for treatment. Two markers, identified by mass spectrometry analysis as α1-antitrypsin and NS1 proteins were found to be upregulated in dengue fever (DF; n = 10) and dengue haemorrhagic fever (DHF; n = 10) patients compared with healthy individuals ( n = 8). Both α1-antitrypsin and NS1 proteins were overexpressed two-fold in DHF patients compared with DF patients. Our study suggests that α1-antitrypsin and NS1 protein could be used as biomarkers as early indicators of DHF risk among patients with suspected dengue infection.