microRNA profile datasets of murine macrophages infected with different strains of Leptospira spp

• Published in: Scientific data Vol. 5; no. 1; p. 180171
• Main Authors: Garcia, Leandro E., Junior, Erivelto C. A., Bragato, Jaqueline P., Melo, Larissa M., Lima, Valéria F. M., Peiró, Juliana R., Arnold, Daniel R., Marinho, Márcia, Lopes, Flavia L.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 21.08.2018; Nature Publishing Group
• Subjects: 631/208/176/2016; 692/420/254; Animals; Data Descriptor; DNA microarrays; Gene expression; Gene Expression Regulation; Gene regulation; Host-pathogen interactions; Humanities and Social Sciences; Immune response; Leptospira; Leptospira - classification; Leptospira - genetics; Leptospirosis; Leptospirosis - genetics; Leptospirosis - microbiology; Macrophages; Macrophages - metabolism; Macrophages - microbiology; Mice; MicroRNAs - biosynthesis; MicroRNAs - genetics; miRNA; Morbidity; multidisciplinary; Pathogenicity; Pathogens; Post-transcription; Science; Species Specificity; Strains (organisms); Zoonoses
• ISSN: 2052-4463; 2052-4463
• DOI: 10.1038/sdata.2018.171

MicroRNAs play an important role in the regulation of immune responses. The influence of epigenetic mechanisms, particularly RNA-mediated post-transcriptional regulation of host immune responses has been proven vital following infections by different pathogens, and bacteria can modulated host miRNAs. Global miRNA expression analysis from macrophages infected in vitro with different strains of Leptospira spp was performed using miRNA 4.1 microarray strips. Leptospirosis is a bacterial zoonosis of global importance, responsible for significant morbidity and mortality worldwide. Despite considerable advances, much is yet to be discovered about disease pathogenicity, particularly in regards to host-pathogen interactions. We present here a high-quality dataset examining the microtranscriptome of murine macrophages J774A.1 following 8h of infection with virulent, attenuated and saprophyte strains of Leptospira . Metadata files were submitted to the Gene Expression Omnibus (GEO) repository. Design Type(s) parallel group design • strain comparison design • microRNA profiling by array design Measurement Type(s) microRNA profiling assay Technology Type(s) microarray platform Factor Type(s) Leptospira • biological replicate Sample Characteristic(s) Mus musculus • macrophage cell line Machine-accessible metadata file describing the reported data (ISA-Tab format)