Class I major histocompatibility complex cDNA clones from sheep thymus: alternative splicing could make a long cytoplasmic tail

To investigate the class I major histocompatibility complex (MHC) genes expressed in the young sheep thymus, a cDNA library was screened with a human HLA-B7 cDNA probe under conditions of relaxed stringency. Thirteen clones were isolated and found by partial sequences to fall into five classes, requ...

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Bibliographic Details
Published inImmunogenetics (New York) Vol. 32; no. 2; p. 77
Main Authors Grossberger, D, Hein, W, Marcuz, A
Format Journal Article
LanguageEnglish
Published United States 01.08.1990
Subjects
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
Gene Library
Major Histocompatibility Complex - genetics
Molecular Sequence Data
RNA Splicing
Sequence Homology, Nucleic Acid
Sheep - genetics
Thymus Gland
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Summary:To investigate the class I major histocompatibility complex (MHC) genes expressed in the young sheep thymus, a cDNA library was screened with a human HLA-B7 cDNA probe under conditions of relaxed stringency. Thirteen clones were isolated and found by partial sequences to fall into five classes, requiring the expression of at least three loci. One sequence was found six times, almost half of the total, and may thus represent the major message expressed in the young sheep thymus. One of the clones was found to have failed to excise the intron between cytoplasmic exons 7 and 8, leading to the predicted synthesis of a cytoplasmic domain 23 amino acids longer than the other sheep sequences, and 15 amino acids longer than any cytoplasmic domain previously described. The sequences of all the clones were found to be most similar to bovine, and least similar to mouse class I MHC sequences.
ISSN:0093-7711
DOI:10.1007/BF00210444