AIEgen based light-up probes for live cell imaging

• Published in: Science China. Chemistry Vol. 59; no. 1; pp. 53 - 61
• Main Authors: Liang, Jing, Feng, Guangxue, Kwok, Ryan Tsz Kin, Ding, Dan, Tang, Benzhong, Liu, Bin
• Format: Journal Article
• Language: English
• Published: Beijing Science China Press 2016; Springer Nature B.V
• Subjects: Biomarkers; Cell membranes; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Fluorescence; HT-29细胞; Imaging; Signal to noise ratio; 乙烯装置; 发光探针; 基础; 核定位信号; 活细胞成像; 生物标志物; 细胞膜; targeted cell imaging; live cell imaging; nucleus imaging; AIE probes; aggregation-induced emission (AIE); light-up probe
• ISSN: 1674-7291; 1869-1870
• DOI: 10.1007/s11426-015-5470-2

Fluorescent light-up probes comprising a tetraphenylethene unit with aggregation-induced emission（AIE）characteristics and a water-soluble peptide have been designed and synthesized which provide cell membrane and nuclear permeability to live cells.This strategy has offered new opportunities for the development of probes with light-up ability and good signal-to-noise ratio.The selectivity or targeting specificity is determined by the peptide sequence,i.e.a nuclear localization signal that leads to nucleus imaging and a cell biomarker targeting peptide that offers specific light-up imaging of HT-29 cells.