Volumetric absorptive microsampling as an effective microsampling technique for LC–MS/MS bioanalysis of biomarkers in drug discovery

• Published in: Bioanalysis Vol. 15; no. 14; pp. 845 - 859
• Main Authors: Kapadnis, Unnati, Locuson, Chuck, Okamura, Heidi, Rienzo, Gianluca De, Cotter, Cece, Zhu, Dongwei, Narayanaswami, Rohini, Castro-Perez, Jose, Marathe, Punit, Yang, Wen-Chu
• Format: Journal Article
• Language: English
• Published: England Newlands Press Ltd 01.07.2023
• Subjects: amino acids; biomarkers; carboxylic acids; LC–MS/MS; microsampling; VAMS; VAMS; microsampling; LC–MS/MS; biomarkers; amino acids; carboxylic acids
• ISSN: 1757-6180; 1757-6199
• DOI: 10.4155/bio-2023-0059

Develop and validate a volumetric absorptive microsampling (VAMS)-based LC–MS/MS method to support the bioanalysis of amino acid and carboxylic acid biomarkers in mouse whole blood. Mouse whole blood was collected using a 10 μl VAMS device. The analytes in VAMS were extracted and analyzed using an LC–MS/MS method. The VAMS-based LC–MS/MS assay exhibited a linearity range of 10.0–10,000 ng/ml with acceptable precision and accuracy and consistent recovery. The analyte stability in mouse whole blood VAMS was shown for 7 days at ambient conditions and at -80°C, as well as with three freeze/thaw cycles. A simple and robust VAMS-based LC–MS/MS method was developed and further validated for simultaneous bioanalysis of nine biomarkers in mouse whole blood.