A new approach to purification of bacterial cellulose membranes: What happens to bacteria in supercritical media?

[Display omitted] •Bacterial cellulose membranes can be purified in supercritical media.•Processing of BC films in scCO2 provides the necessary sterility.•Inactivated bacteria preserve the morphology after processing in supercritical fluids. In this paper, we report our attempts to elaborate the met...

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Bibliographic Details
Published inThe Journal of supercritical fluids Vol. 147; pp. 59 - 69
Main Authors Pigaleva, Marina A., Bulat, Matvey V., Gromovykh, Tatiana I., Gavryushina, Irina A., Lutsenko, Sergey V., Gallyamov, Marat O., Novikov, Ilya V., Buyanovskaya, Anastasiia G., Kiselyova, Olga I.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.05.2019
Subjects
Atomic force microscopy
Bacterial cellulose
Endotoxins
Gluconacetobacter hansenii
Supercritical processing
BC
Atomic force microscopy
SDS
Gluconacetobacter hansenii
Endotoxins
AFM
Supercritical processing
LAL test
H5
LPS
scCO2
FTIR
Bacterial cellulose
SEM
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Summary:[Display omitted] •Bacterial cellulose membranes can be purified in supercritical media.•Processing of BC films in scCO2 provides the necessary sterility.•Inactivated bacteria preserve the morphology after processing in supercritical fluids. In this paper, we report our attempts to elaborate the method of purification and simultaneous sterilization of bacterial cellulose films by means of processing them under high pressure, including supercritical conditions for carbon dioxide, for future application in medicine. Bacterial cellulose films were produced by Gluconacetobacter hansenii. The processed samples were controlled by means of AFM, SEM, EDS, IR spectrometry, elemental and microbiological analysis. It was shown that conventional methods do not always provide complete removal of cells and endotoxins and the samples contain traces of compounds used for washing. It was revealed that the outer lipopolysaccharide membrane of the producent cells was destroyed during processing, but bacterial cells still preserved their shape and dimensions. The processed samples were sterile; the level of endotoxins was strongly reduced, though, unfortunately the degree of purification required for medical use was not achieved.
ISSN:0896-8446
1872-8162
DOI:10.1016/j.supflu.2019.02.009