Correlation of (S+G2)-phase fractions determined by flow cytometry and Ki-67 labelling indices in colorectal carcinomas

Samples from 14 human colorectal carcinomas were analysed by means of classical (one-parameter) flow cytometry. (S+G2)-phase fractions were calculated from DNA histograms with the help of two different algorithms. At the same time, the percentage of cells positive for the Ki-67 antibody, which recog...

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Bibliographic Details
Published inOncology Vol. 51; no. 1; p. 9
Main Authors Zölzer, F, Gilbertz, K, Streffer, C
Format Journal Article
LanguageEnglish
Published Switzerland 01.01.1994
Subjects
Aged
Aged, 80 and over
Algorithms
Colorectal Neoplasms - pathology
Colorectal Neoplasms - surgery
DNA, Neoplasm - analysis
Female
Flow Cytometry - methods
G2 Phase
Humans
Ki-67 Antigen
Male
Middle Aged
Mitotic Index
Neoplasm Proteins - analysis
Nuclear Proteins - analysis
S Phase
Tumor Cells, Cultured - pathology
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Summary:Samples from 14 human colorectal carcinomas were analysed by means of classical (one-parameter) flow cytometry. (S+G2)-phase fractions were calculated from DNA histograms with the help of two different algorithms. At the same time, the percentage of cells positive for the Ki-67 antibody, which recognizes proliferating cells, was determined in cytocentrifuge preparations. Significant correlations between the two indices were found only if the histogram analysis included background subtraction and aggregate correction. This finding may throw some light on the reliability of prognosis based on flow cytometry.
ISSN:0030-2414
DOI:10.1159/000227301