Two-step salting-out extraction of 1,3-propanediol, butyric acid and acetic acid from fermentation broths

• Published in: Separation and purification technology Vol. 209; pp. 246 - 253
• Main Authors: Li, Zhen, Yan, Ling, Zhou, Jinjie, Wang, Xiaoli, Sun, Yaqin, Xiu, Zhi-Long
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 31.01.2019
• Subjects: 1,3-Propanediol; Acetic acid; Back-extraction; Butyric acid; Salting-out extraction; Back-extraction; 1,3-Propanediol; Acetic acid; Salting-out extraction; Butyric acid
• ISSN: 1383-5866; 1873-3794
• DOI: 10.1016/j.seppur.2018.07.021

•A novel two-step salting-out extraction was used to separate 1,3-propanediol, butyric acid and acetic acid from fermentation broth.•Butyric acid could be separated from 1,3-propanediol and acetic acid by the salting-out extraction system comprised of acidic inorganic salt and hydrophobic solvent.•Effect of sodium carbonate concentration on the back-extraction of butyric acid was investigated. The separation of 1,3-propanediol (1,3-PD), butyric acid (BA) and acetic acid (HAc) from the fermentation broth was studied by two-step salting-out extraction. In the first salting-out extraction, the partition coefficient and recovery of BA reached 42.21 and 96.42%, respectively, under the optimal condition of 25 wt% NaH2PO4/30 wt% n-butyl acetate. Subsequently, 91.28% of BA in the organic phase could be recovered through back-extraction when sodium carbonate solution was mixed with organic phase with initial phase ratio of n-butyl acetate solution to alkaline solution being 2:1, in which the molar ratio of sodium carbonate to organic acids was 3:5. Finally, 50% (v/v) ethanol (95%) was added to the bottom phase for the second step salting-out extraction. And the partition coefficient and recovery of 1,3-PD were 9.40 and 95.50%, and those of HAc were 7.46 and 94.40%, respectively. All the cells and most of the proteins (97.16%) could be removed. Effective separation of 1,3-PD from BA was realized by two-step salting-out extraction, which provides a potential method for separation of 1,3-PD, BA and HAc on an industrial scale.