Sub-cellular precision on-chip small-animal immobilization, multi-photon imaging and femtosecond-laser manipulation

Techniques for stable, rapid and repeatable small-animal immobilization are necessary for high-throughput in vivo genetic/drug screens using cellular and sub-cellular features in multi-cellular organisms. We demonstrate a method for non-invasive and high-throughput on-chip immobilization of physiolo...

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Bibliographic Details
Published inLab on a chip Vol. 8; no. 5; pp. 653 - 656
Main Authors Zeng, Fei, Rohde, Christopher B, Yanik, Mehmet Fatih
Format Journal Article
LanguageEnglish
Published England 01.01.2008
Subjects
Animals
Caenorhabditis elegans - physiology
Caenorhabditis elegans - ultrastructure
Immobilization - instrumentation
Immobilization - methods
Lasers
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Microscopy, Fluorescence, Multiphoton - instrumentation
Microscopy, Fluorescence, Multiphoton - methods
Sensitivity and Specificity
Subcellular Fractions - physiology
Subcellular Fractions - ultrastructure
Time Factors
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Summary:Techniques for stable, rapid and repeatable small-animal immobilization are necessary for high-throughput in vivo genetic/drug screens using cellular and sub-cellular features in multi-cellular organisms. We demonstrate a method for non-invasive and high-throughput on-chip immobilization of physiologically active C. elegans without the use of anesthesia or cooling, but with comparable stability even for the most demanding purposes. We show observation and manipulation of sub-cellular features in immobilized animals using two-photon microscopy and femtosecond-laser microsurgery.
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ISSN:1473-0197
1473-0189
DOI:10.1039/b804808h