Compositional study of gallbladder stone using photoacoustic spectroscopy

• Published in: Optics and laser technology Vol. 111; pp. 696 - 700
• Main Authors: Gazali, Zainab, Thakur, S.N., Rai, A.K.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.04.2019; Elsevier BV
• Subjects: Absorption spectra; Acetone; Biomedical materials; Calcium carbonate; Chemical compounds; Cholesterol; Gallbladder; Gallstones; Laser induced breakdown spectroscopy; Organic chemistry; Photoacoustic spectroscopy; Spectrum analysis; Photoacoustic Spectroscopy (PAS); UV–Visible absorption; Gallbladder stone; LIBS; Cholesterol; Calcium carbonate
• ISSN: 0030-3992; 1879-2545
• DOI: 10.1016/j.optlastec.2018.09.003

•Study of chemical compounds without any sample preparation is performed using PAS.•Molecular analysis of gallstones using PAS has been done for the first time.•The results of PAS are validated with results of UV–Visible and LIBS.•Presence of Cholesterol, Calcium carbonate and Bile acid are confirmed by PAS.•Elemental profile (C, Ca, Mg etc) of gallstone are confirm using LIBS. Molecular composition of gallbladder stone has been investigated for the first time by means of photoacoustic spectroscopy (PAS). The presence of cholesterol, calcium carbonate and bile acid, in the photoacoustic spectrum, have been established and compared with the UV–Visible absorption spectrum of acetone solution of powdered gallstone. The results of this investigation show that PAS is better suited to detect the presence of different chemical compounds in gallstones as compared to conventional absorption spectroscopy. Moreover, PAS does not require elaborate biomaterial’s sample preparation. Laser induced breakdown spectroscopy (LIBS) has been applied to reveal presence of atomic species in gallstone, that are involved in the molecular constituents revealed by PAS.