Three distinct regions of deletion on 13q in squamous cell carcinoma of the larynx

Genetic etiology of squamous cell carcinoma of the larynx (SCCL) is very complex, with both molecular and chromosomal alterations involved. The target genes have not yet been clearly identified. Therefore, our study focused on searching for regions that potentially harbor genes related to SCCL. Afte...

Full description

Saved in:
Bibliographic Details
Published inOncology reports Vol. 16; no. 2; pp. 417 - 421
Main Authors STEMBALSKA, Agnieszka, BLIN, Nikolaus, RAMSEY, David, SASIADEK, Maria M
Format Journal Article
LanguageEnglish
Published Athens S.n. 01.08.2006
Subjects
Biological and medical sciences
Carcinoma, Squamous Cell - genetics
Chromosomes, Human, Pair 13 - genetics
DNA, Neoplasm - analysis
Humans
Laryngeal Neoplasms - genetics
Loss of Heterozygosity
Medical sciences
Microsatellite Repeats
Nucleic Acid Hybridization
Otorhinolaryngology. Stomatology
Sequence Deletion
Tumors
Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology
Laryngeal cancer
Allelic imbalance
Chromosome D13
chromosome 13q
Malignant tumor
Gene expression
Larynx squamous cell carcinoma
Cancerology
Comparative genomic hybridization
Loss of heterozygosity
Deletion
ENT disease
Genetics
Larynx disease
Online AccessGet full text

Cover

More Information
Summary:Genetic etiology of squamous cell carcinoma of the larynx (SCCL) is very complex, with both molecular and chromosomal alterations involved. The target genes have not yet been clearly identified. Therefore, our study focused on searching for regions that potentially harbor genes related to SCCL. After comparative genomic hybridization (CGH) analysis of a set of 52 SCCL we specified 13q21-q32 and 13q34 as the most frequently deleted regions. In order to precisely map the critical region of deletion, we studied these areas by using 15 microsatellite markers. In our material a significantly high frequency of loss of heterozygosity (LOH) (test for a difference in two proportions, p<0.001) was observed for the following markers: D13S1320 (13q21.1), D13S800 (13q21.3), D13S1818 (13q32.1), D13S770 (13q32.3) and D13S285 (13q34). Three hot spots of LOH were found: 13q21.1-q22.1 (D13S1320-D13S1824-D13S800-SHGC30014- WI-16413-D13S1186), 13q.31.1-13q32.3 (D13S317-D13S1818-D13S770), 13q34 (D13S285). Among these areas, 13q31.1-q32.3 was identified as new hot spot of deletion in SCCLs.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1021-335X
1791-2431
DOI:10.3892/or.16.2.417