The anti-inflammatory, anti-oxidant and protective effects of a probiotic mixture on organ toxicity in a rat model

• Published in: Future microbiology Vol. 15; no. 6; pp. 401 - 412
• Main Authors: Karamese, Murat, Aydin, Hakan, Gelen, Volkan, Sengul, Emin, Karamese, Selina Aksak
• Format: Journal Article
• Language: English
• Published: England Future Medicine Ltd 01.04.2020
• Subjects: inflammation; liver and spleen damage; NF-κB; probiotics; NF-κB; liver and spleen damage; inflammation; probiotics
• ISSN: 1746-0913; 1746-0921
• DOI: 10.2217/fmb-2020-0005

The objective of this study was to evaluate the possible protective effects of probiotic bacteria, especially and strains, on 4,4′-dichlorodiphenyltrichloroethane (DDT)-induced toxicity. For this reason, we evaluated the relationship between probiotics and toxicity by checking immunological and immunohistochemical parameters. Probiotic pretreatment was applied to 36 Wistar albino rats for 12 consecutive days. Serum aspartate aminotransferase and alanine aminotransferase levels were detected. CD3 and NF-κB staining methods were then performed by immunohistochemistry. Finally, pro- and anti-inflammatory cytokines were measured by ELISA. DDT caused a serious increase/decrease in some cytokine parameters. The effective dose was 1 × 10  colony-forming unit probiotic treatment. CD3 and NF-κB positivity were intense in DDT group whereas the intensity was reduced in probiotic treatment groups. The probiotic mixture has a potential to prevent inflammatory and oxidative stress related organ injuries. Further studies should be performed to explain the possible mechanisms.