Linear and branched polyacrylates as a delivery platform for peptide-based vaccines

Peptide-based vaccines are designed to carry the minimum required antigen to trigger the desired immune responses; however, they are usually poorly immunogenic and require appropriate delivery system. Peptides, B-cell epitope (J14) derived from group A streptococcus M-protein and universal T-helper...

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Bibliographic Details
Published inTherapeutic delivery Vol. 7; no. 9; pp. 601 - 609
Main Authors Chandrudu, Saranya, Bartlett, Stacey, Khalil, Zeinab G, Jia, Zhongfan, Hussein, Waleed M, Capon, Robert J, Batzloff, Michael R, Good, Michael F, Monteiro, Michael J, Skwarczynski, Mariusz, Toth, Istvan
Format Journal Article
LanguageEnglish
Published England Future Science Ltd 01.09.2016
Subjects
Acrylic Resins - chemistry
Animals
Antigens, Bacterial - administration & dosage
Bacterial Outer Membrane Proteins - administration & dosage
Carrier Proteins - administration & dosage
clinical isolates
Epitopes, B-Lymphocyte - administration & dosage
Epitopes, T-Lymphocyte - administration & dosage
group A streptococcus
Immunization
Mice
nanoparticles
opsonization
peptide vaccine
Peptides - administration & dosage
polyacrylates
polymer-peptide conjugate
single immunization
vaccine delivery
Vaccines, Subunit - administration & dosage
vaccine delivery
nanoparticles
polymer–peptide conjugate
polyacrylates
group A streptococcus
opsonization
clinical isolates
peptide vaccine
single immunization
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Summary:Peptide-based vaccines are designed to carry the minimum required antigen to trigger the desired immune responses; however, they are usually poorly immunogenic and require appropriate delivery system. Peptides, B-cell epitope (J14) derived from group A streptococcus M-protein and universal T-helper (PADRE) epitope, were conjugated to a variety of linear and branched polyacrylates. All produced conjugates formed submicron-sized particles and induced a high level of IgG titres in mice after subcutaneous immunization. These polymer-peptide conjugates demonstrated high opsonization capacity against group A streptococcus clinical isolates. We have successfully demonstrated that submicron-sized polymer-peptide conjugates were capable of inducing strong humoral immune responses after single immunization.
ISSN:2041-5990
2041-6008
DOI:10.4155/tde-2016-0037