Release of met-enkephalin and its modulation through acetylcholine receptors in the rabbit superior colliculus

This report presents evidence for the depolarization-dependent release of met-enkephalin from the superior colliculus of the rabbit. Collicular tissue was placed in superfusion chambers and met-enkephalin accumulation in the superfusate was measured by radioimmunoassay. Exposure to high potassium co...

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Bibliographic Details
Published inExperimental brain research Vol. 82; no. 3; p. 663
Main Authors Illing, R B, Nikolarakis, K E, Wichmann, T, Spatz, W B, Starke, K
Format Journal Article
LanguageEnglish
Published Germany 01.01.1990
Subjects
Animals
Atropine - pharmacology
Enkephalin, Methionine - metabolism
Female
Hexamethonium
Hexamethonium Compounds - pharmacology
Kinetics
Male
Potassium - pharmacology
Rabbits
Radioimmunoassay
Receptors, Cholinergic - drug effects
Receptors, Cholinergic - physiology
Superior Colliculi - drug effects
Superior Colliculi - physiology
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Summary:This report presents evidence for the depolarization-dependent release of met-enkephalin from the superior colliculus of the rabbit. Collicular tissue was placed in superfusion chambers and met-enkephalin accumulation in the superfusate was measured by radioimmunoassay. Exposure to high potassium concentrations (30 mM and 56 mM) increased met-enkephalin release. This is the fourth transmitter shown to be released from collicular tissue. Furthermore, we have obtained the first evidence that suggests that met-enkephalin release is susceptible to muscarinic modulation. While the depolarization-dependent release of met-enkephalin was depressed in the presence of atropine (1 microM), hexamethonium (100 microM) did not block the increase of met-enkephalin release induced by high potassium.
ISSN:0014-4819
DOI:10.1007/BF00228809