Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems

• Published in: Molecular & general genetics Vol. 258; no. 1/2; pp. 1 - 8
• Main Authors: Rossi, M, Carrari, F, Cabrera-Ponce, J.L, Vazquez-Rovere, C, Herrera-Estrella, L, Gudesblat, G, Iusem, N.D
• Format: Journal Article
• Language: English
• Published: Germany 01.04.1998
• Subjects: abscisic acid; Abscisic Acid - pharmacology; Agrobacterium tumefaciens; asr2 promoter; beta-glucuronidase; biolistics; callus; Carica papaya; gene expression; Gene Expression Regulation; gene transfer; genetic regulation; Glucuronidase - genetics; histochemistry; leaves; Lycopersicon esculentum - genetics; multigene family; Nicotiana tabacum; Plant Proteins - genetics; Plants, Genetically Modified; promoter regions; Promoter Regions, Genetic; recombinant DNA; Recombinant Fusion Proteins - biosynthesis; reporter genes; Solanum lycopersicum var. lycopersicum; Solanum tuberosum; transgenic plants
• ISSN: 0026-8925; 1432-1874
• DOI: 10.1007/s004380050700

Asr is a family of genes that maps to chromosome 4 of tomato. Asr2, a recently reported member of this family, is believed to be regulated by abscisic acid (ABA), stress and ripening. A genomic Asr2 clone has been fully sequenced, and candidate upstream regulatory elements have been identified. To prove that the promoter region is functional in vivo, we fused it upstream of the beta-glucuronidase (GUS) reporter gene. The resulting chimeric gene fusion was used for transient expression assays in papaya embryogenic calli and leaves. In addition, the same construct was used to produce transgenic tomato, papaya, tobacco, and potato plants. Asr2 upstream sequences showed promoter function in all of these systems. Under the experimental conditions tested, ABA stimulated GUS expression in papaya and tobacco, but not in tomato and potato systems.