Noninvasive imaging of radiolabeled exosome-mimetic nanovesicle using 99mTc-HMPAO
Abstract Exosomes known as nano-sized extracellular vesicles attracted recent interests due to their potential usefulness in drug delivery. Amid remarkable advances in biomedical applications of exosomes, it is crucial to understand in vivo distribution and behavior of exosomes. Here, we developed a...
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Published in | Scientific reports Vol. 5; no. 1; p. 15636 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
London
Nature Publishing Group
26.10.2015
|
Subjects | |
Online Access | Get full text |
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Summary: | Abstract
Exosomes known as nano-sized extracellular vesicles attracted recent interests due to their potential usefulness in drug delivery. Amid remarkable advances in biomedical applications of exosomes, it is crucial to understand
in vivo
distribution and behavior of exosomes. Here, we developed a simple method for radiolabeling of macrophage-derived exosome-mimetic nanovesicles (ENVs) with
99m
Tc-HMPAO under physiologic conditions and monitored
in vivo
distribution of
99m
Tc-HMPAO-ENVs using SPECT/CT in living mice. ENVs were produced from the mouse RAW264.7 macrophage cell line and labeled with
99m
Tc-HMPAO for 1 hr incubation, followed by removal of free
99m
Tc-HMPAO. SPECT/CT images were serially acquired after intravenous injection to BALB/c mouse. When ENVs were labeled with
99m
Tc-HMPAO, the radiochemical purity of
99m
Tc-HMPAO-ENVs was higher than 90% and the expression of exosome specific protein (CD63) did not change in
99m
Tc-HMPAO-ENVs.
99m
Tc-HMPAO-ENVs showed high serum stability (90%) which was similar to that in phosphate buffered saline until 5 hr. SPECT/CT images of the mice injected with
99m
Tc-HMPAO-ENVs exhibited higher uptake in liver and no uptake in brain, whereas mice injected with
99m
Tc-HMPAO showed high brain uptake until 5 hr. Our noninvasive imaging of radiolabeled-ENVs promises better understanding of the
in vivo
behavior of exosomes for upcoming biomedical application. |
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Bibliography: | These authors contributed equally to this work. |
ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/srep15636 |