Whole Mammary Gland Transplantation in Mice Protocol

• Published in: Bio-protocol Vol. 7; no. 11
• Main Authors: Hansford, Hayley, Hong, Yan, Kittrell, Frances, Medina, Daniel, Behbod, Fariba
• Format: Journal Article
• Language: English
• Published: United States Bio-Protocol 05.06.2017; Bio-protocol LLC
• Subjects: Biology; Methods; Mammary tumorigenesis; Viable epithelial cells; Mammary gland; Transplant
• ISSN: 2331-8325; 2331-8325
• DOI: 10.21769/BioProtoc.2326

Whole Mammary Gland Transplantation involves transplanting an excised mammary gland into another, more suitable host. This method can be used to extend the life of a mammary gland past the mouse's life span by transplanting the mammary gland of an older mouse into a young healthy mouse. As you can see in the video below (Video 1), by attaching it to the abdomen of the mouse, the gland will receive a steady blood supply and both epithelial and stromal cells will remain viable for up to one year. Although this method is not used often, it has been part of several experiments including determining whether the stroma or epithelium is the primary target in chemically induced mouse mammary tumorigenesis (Medina and Kittrell, 2005). To monitor transplants, palpate every week for tumor formation. The transplanted mammary gland may also be passaged serially every 8-10 weeks. Keep transplanted gland in the same mouse for no longer than one year.