Effect of sleep deprivation on serum influenza-specific IgG

• Published in: Sleep (New York, N.Y.) Vol. 21; no. 1; pp. 19 - 24
• Main Authors: RENEGAR, K. B, FLOYD, R, KRUEGER, J. M
• Format: Journal Article
• Language: English
• Published: Rochester, MN American Academy of Sleep Medicine 1998
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antibodies, Viral - immunology; Antibodies, Viral - metabolism; Behavioral psychophysiology; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Immunization, Passive; Immunoglobulin G - blood; Immunoglobulin G - immunology; Immunoglobulin G - metabolism; Immunology and behavior; Mice; Mice, Inbred BALB C; Orthomyxoviridae Infections - blood; Orthomyxoviridae Infections - immunology; Orthomyxoviridae Infections - metabolism; Psychology. Psychoanalysis. Psychiatry; Psychology. Psychophysiology; Sleep Deprivation - physiology; Time Factors; Vaccination; Vigilance; Deprivation; Immune response; Rodentia; IgG; Orthomyxoviridae; Influenzavirus; Infection; Virus; Vertebrata; Mammalia; Sleep; Mouse; Viral disease; Animal; Influenza; Serum
• ISSN: 0161-8105; 1550-9109; 1550-9109
• DOI: 10.1093/sleep/21.1.19

Intra-pulmonary protection against influenza virus in immune mice, largely dependent upon serum lgG, is reported to be suppressed by 7 hours of sleep deprivation following viral challenge. This implies that sleep deprivation may accelerate the catabolism of influenza-specific antibodies. To determine the effects of sleep deprivation on the catabolism of serum antibodies, BALB/c mice were passively immunized intravenously with lgG anti-influenza monoclonalantibodies and catabolism kinetics monitored for 6 days. Mice were then sleep-deprived for either 9 hours (one episode) or for 9 hours followed by 6 hours on the consecutive day (two episodes) and the serum titer of influenza-specific monoclonal antibodies monitored for an additional 8 days via ELISA. One episode of sleep-deprivation had only minor effects on lgG catabolism; however, two episodes of sleep-deprivation caused significant changes in the kinetics of lgG catabolism, resulting in elevated lgG levels (p = 0.02) for 2 days post-sleep deprivation. Elevation of serum influenza-specific lgG (p = 0.005) was also seen in actively immune mice following two episodes of sleep-deprivation. Serum chemistries ruled out dehydration as a cause of the increased antibody levels; however, some anomalies were noted: total protein and albumin were elevated, although not significantly, and P and Ca were decreased. Thus, our data do not support the hypothesis that sleep-deprivation lowers existing serum antibody titers by accelerating antibody catabolism.