6′-Sialyllactose Alleviates Muscle Fatigue through Reduced Blood Lactate Level after Treadmill Exercise in Mice

• Published in: Nutrients Vol. 16; no. 17; p. 2957
• Main Authors: Park, Eun-Jung, Kim, Li-La, Lee, Jie-Oh, Lee, Hay-Young, Kim, Yong-An, Go, Hiroe
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 03.09.2024
• Subjects: Animals; Biomarkers; blood glucose; Blood Glucose - drug effects; Blood Glucose - metabolism; blood serum; brain; breast milk; Cytochrome; digestive system; E coli; Endurance; Enzymes; Exercise; Fitness equipment; Food; food intake; Glucose; Kinases; Laboratories; lactic acid; Lactic Acid - blood; Lactose - analogs & derivatives; Lactose - pharmacology; lipids; Male; males; Metabolism; Mice; Mice, Inbred C57BL; Mitochondria; Muscle fatigue; Muscle Fatigue - drug effects; muscle fibers; Muscle, Skeletal - drug effects; Muscle, Skeletal - metabolism; muscles; oxidative phosphorylation; Physical Conditioning, Animal; Physiology; Production increases; protein synthesis; Proteins; Software; United States > US; South Korea; Japan; oxidative phosphorylation protein complexes; anti-fatigue; muscle fiber type; lactate; 6′-sialyllactose
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu16172957

6′-Sialyllactose (6′-SL), found in human breast milk, exhibits anti-inflammatory, immune function-enhancing, brain development-promoting, and gut health-improving effects. However, its effects on muscle fatigue remain unknown. Here, we aimed to investigate the effects of 6′-SL on blood lactate level, muscle fiber type, and oxidative phosphorylation protein complexes (OXPHOS) in muscle after exercise using C57BL/6J male mice. C57BL/6J mice were randomly assigned to control or 100 mg/kg 6′-SL. After 12 weeks of 6′-SL administration, the mice were made to perform treadmill exercise; their blood lactate and glucose levels were measured at the basal level (rest) and 0, 5, and 10 min after treadmill exercise. Results showed that 6′-SL treatment in C57BL/6J mice significantly reduced blood lactate level and improved blood glucose level. Moreover, 6′-SL increased the expression of slow-myosin heavy chain (MHC) and OXPHOS in gastrocnemius muscle. In addition, 6′-SL treatment for 12 weeks did not affect food intake, serum biomarkers of tissue injury, and lipid profiles compared with those of the controls. These findings indicate that non-toxic 6′-SL suppressed muscle fatigue during exercise by promoting protein expression of muscle fibers, especially slow-twitch muscle fibers characterized by abundant OXPHOS complexes and decreased blood lactate level. This study suggests that 6′-SL holds promise as a nutritional supplement in exercise and clinical settings, subject to further validation.