Five-wavelength optical-resolution photoacoustic microscopy of blood and lymphatic vessels

Optical-resolution photoacoustic microscopy (OR-PAM) has been developed for anatomical, functional, and molecular imaging but usually requires multiple scanning for different contrasts. We present five-wavelength OR-PAM for simultaneous imaging of hemoglobin concentration, oxygen saturation, blood f...

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Bibliographic Details
Published inAdvanced photonics Vol. 3; no. 1; p. 016002
Main Authors Liu, Chao, Chen, Jiangbo, Zhang, Yachao, Zhu, Jingyi, Wang, Lidai
Format Journal Article
LanguageEnglish
Published Bellingham Society of Photo-Optical Instrumentation Engineers 01.01.2021
S P I E - International Society for
Subjects
Blood flow
Blood vessels
Efficiency
Energy
Hemoglobin
Image acquisition
Lasers
Medical imaging
Microscopy
Optics
Oxygen
Oxygen content
Oxygen saturation
Ozone
Photoacoustic microscopy
Physiology
Pulsed lasers
Raman spectra
Raster scanning
Wavelengths
stimulated Raman scattering
single-shot
multi-contrast microscopy
early cancer detection
optical-resolution photoacoustic microscopy
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Summary:Optical-resolution photoacoustic microscopy (OR-PAM) has been developed for anatomical, functional, and molecular imaging but usually requires multiple scanning for different contrasts. We present five-wavelength OR-PAM for simultaneous imaging of hemoglobin concentration, oxygen saturation, blood flow speed, and lymphatic vessels in single raster scanning. We develop a five-wavelength pulsed laser via stimulated Raman scattering. The five pulsed wavelengths, i.e., 532, 545, 558, 570, and 620  /  640  nm, are temporally separated by several hundreds of nanoseconds via different optical delays in fiber. Five photoacoustic images at these wavelengths are simultaneously acquired in a single scanning. The 532- and 620  /  640-nm wavelengths are used to image the blood vessels and dye-labeled lymphatic vessels. The blood flow speed is measured by a dual-pulse method. The oxygen saturation is calculated and compensated for by the Grüneisen-relaxation effect. In vivo imaging of hemoglobin concentration, oxygen saturation, blood flow speed, and lymphatic vessels is demonstrated in preclinical applications of cancer detection, lymphatic clearance monitoring, and functional brain imaging.
ISSN:2577-5421
2577-5421
DOI:10.1117/1.AP.3.1.016002