Production kinetics of acidophilin 801, a bacteriocin produced by Lactobacillus acidophilus IBB 801

• Published in: FEMS microbiology letters Vol. 190; no. 2; pp. 305 - 308
• Main Authors: Zamfir, M, Callewaert, R, Cornea, P.C, Vuyst, L. de
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.09.2000; Blackwell; Oxford University Press
• Subjects: Acidophilin 801; adsorption; Bacteriocin; Bacteriocins; Bacteriocins - biosynthesis; Bacteriology; Biological and medical sciences; Biosynthesis; Controlled conditions; Culture Media; Fundamental and applied biological sciences. Psychology; Kinetics; Lactic acid; Lactobacillus acidophilus; Lactobacillus acidophilus - growth & development; Lactobacillus acidophilus - metabolism; Metabolites; microbial growth; Microbiology; Microorganisms; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Production kinetics; Stationary phase; Yogurt; Production kinetics; Acidophilin 801; Bacteriocin; Lactic acid bacteria; Bacteria; Lactobacillaceae; Biosynthesis; Kinetics; Lactobacillus acidophilus
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.2000.tb09303.x

Lactobacillus acidophilus IBB 801 produces a small bacteriocin, designated acidophilin 801. Studying the relationship between growth and bacteriocin biosynthesis revealed primary metabolite kinetics of bacteriocin production with a peak activity at the end of the exponential growth phase followed by a decrease during the stationary phase. Both microbial growth and bacteriocin production was inhibited by lactic acid. Whereas volumetric bacteriocin production (activity units (AU) ml-1) was favoured under pH-controlled conditions, bacteriocin titres rapidly decreased because of strong adsorption of the bacteriocin molecules to the producing cells under less acidic conditions.