Evaluation of Stat Assay of Serum Digoxin Concentration by Radioimmunoassay and Its Application for Digoxin Regimen Based on Pharmacokinetics

• Published in: RADIOISOTOPES Vol. 29; no. 9; pp. 432 - 437
• Main Authors: MASUHARA, Keiso, SASAKI, Yasuhito, TABUSE, Katsuyoshi, KIDA, Hirokazu, SHINOZAKI, Kimikazu, SATO, Akemi, ARAI, Sakae, SAKAKI, Tokuichi, KASHIWADA, Kazuko, SOMEYA, Kazuhiko
• Format: Journal Article
• Language: Japanese
• Published: Japan Japan Radioisotope Association 1980
• Subjects: Aged; Digoxin - blood; Digoxin - metabolism; digoxine intoxication; distribution volume; Heart Failure - blood; Humans; Kinetics; Male; Radioimmunoassay - methods; Reagent Kits, Diagnostic; stat assay
• ISSN: 0033-8303; 1884-4111
• DOI: 10.3769/radioisotopes.29.9_432

Serum digoxin concentrations measured by stat RIA (phadebas digoxin RIA kit) correlated well with results obtained by complete assays. The result of stat assay can be reported within 1 hour, measuring one or more samples together with 2 standard samples in duplicates. Precise measurement can be expected with serum digoxin concentration over 0.5 ng/ml. The stat assay allows to apply the theory of pharmacokinetics for the estimation of digoxin concentration at steady states measuring minimum digoxin concentration (Cn (min) ) on the 3rd to 6th day after the start of digoxin therapy. The estimated serum digoxin levels were well agreed with measured values with the difference ranging from Y.6 to 8.6% in CV. The method is usefull for the planning and assessment of appropriate digoxin regimen.