Legume seed vicilins (7S storage proteins) interfere with the development of the cowpea weevil (Callosobruchus maculatus (F))

• Published in: Journal of the science of food and agriculture Vol. 76; no. 1; pp. 111 - 116
• Main Authors: Yunes, Andréa Neila A, de Andrade, Maria Tereza, Sales, Maurício P, Morais, Rosana A, Fernandes, Kátia Valevski S, Gomes, Valdirene M, Xavier-Filho, José
• Format: Journal Article
• Language: English
• Published: London John Wiley & Sons, Ltd 01.01.1998; Wiley; Published for the Society of Chemical Industry by Elsevier Applied Science
• Subjects: 7S storage proteins; adzuki bean; Biological and medical sciences; Callosobruchus maculatus; Canavalia ensiformis; common bean; cowpea; Fundamental and applied biological sciences. Psychology; Glycine max; Invertebrates; jack bean; legume seeds; lima bean; Medically important nuisances and vectors, pests of stored products and materials: population survey and control; Pests of stored products; Phaseolus lunatus; Phaseolus vulgaris; Phytopathology. Animal pests. Plant and forest protection; Protozoa. Invertebrates; Records, symptoms, damages, economic importance, population surveys; resistance to bruchids; soybean; vicilins; Vigna angularis; Vigna unguiculata; Storage protein; Seeds; Canavalia ensiformis; Phaseolus lunatus; Coleoptera; Insecta; Vigna unguiculata; Pest; Dicotyledones; Insect resistance; Angiospermae; Bruchidae; Development; Callosobruchus maculatus; Glycine max; Biological activity; Grain legume; Leguminosae; Larva; Vigna angularis; Phaseolus vulgaris; Animal plant relation; Arthropoda; Spermatophyta; Invertebrata
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/(SICI)1097-0010(199801)76:1<111::AID-JSFA932>3.0.CO;2-4

Vicilins (7S storage proteins) isolated from the seeds of the legumes Vigna unguiculata (cowpea), Vigna angularis (adzuki bean), Canavalia ensiformis (jack bean), Glycine max (soybean), Phaseolus vulgaris (common bean) and Phaseolus lunatus (lima bean) were shown to be immunologically related and to bind to a chitin matrix. The effect of the isolated vicilins on the development of the cowpea weevil Callosobruchus maculatus was examined. Vicilins from all non‐host seeds, including those of the C maculatus‐resistant cowpea line IT81D‐1045, strongly inhibited larval development (ED50 of 1·07±0·32% to 2·22±0·64%). Vicilins from the C maculatus‐susceptible cowpea CE‐31 and adzuki bean seeds were the exception with ED50 of 6·25±0·75% and 5·40±1·54%, respectively. These results correlate well with the host range of C maculatus and are possibly a reflection of the low digestibility of vicilins by insect midgut proteinases in addition to the ability they show in binding to chitin‐containing structures like the ones found in the bruchid midguts. © 1998 SCI.