Characterization of a Mycobacterium bovis BCG insertion sequence related to the IS21 family

• Published in: FEMS microbiology letters Vol. 178; no. 2; pp. 211 - 217
• Main Authors: Lefèvre, Philippe, Braibant, Martine, Content, Jean, Gilot, Philippe
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.09.1999; Blackwell; Oxford University Press; Wiley-Blackwell
• Subjects: Amino Acid Sequence; Bacillus Calmette-Guerin vaccine; Bacterial Proteins; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacteriology; Base Sequence; BCG; Biological and medical sciences; Corynebacteria‐Mycobacteria‐Nocardia group; DNA Transposable Elements; DNA, Bacterial; DNA, Bacterial - genetics; Fundamental and applied biological sciences. Psychology; Genetics; Genomes; Insertion; IstA; IstB; Life Sciences; Microbiology; Molecular Sequence Data; Mycobacterium bovis; Mycobacterium bovis - genetics; Nucleic acids; Pasteurization; Sequence Alignment; Sequence Analysis, DNA; Tuberculosis; IstA; Corynebacteria-Mycobacteria-Nocardia group; IstB; Mycobacterium bovis; Nucleotide sequence; Sequence alignment; BCG; Gene organization; Transposable element; Open reading frame; IS element; Characterization; Mycobacteriales; Mycobacteriaceae; Bacteria; Actinomycetes; Comparative study; Aminoacid sequence; Structural analysis
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.1999.tb08679.x

Abstract The structure and distribution of a Mycobacterium bovis BCG insertion element of the IS21 family were investigated. Several IS21-like elements found in mycobacterial genomes were separated in four types, following their nucleic acid similarities. The M. bovis BCG IS21 element is highly similar to IS1533 (class I), 70% similar to IS1534 (class II), 52% similar to IS1532 (class III) of Mycobacterium tuberculosis, and 54% similar to both an Mycobacterium avium serovar 2 and an M. avium silvaticum IS (class IV). The M. bovis BCG IS21 element of the class I appears to be present in a single copy in the genome of M. bovis BCG; M. bovis; M. tuberculosis and Mycobacterium africanum and to be absent from all other tested species of the Corynebacteria-Mycobacteria-Nocardia group.