Affinity chromatography with biotinylated RNAs

Small, reversibly biotinylated RNAs as described here are versatile ligands for affinity chromatography of RNA-binding components. These RNAs can be attached to a solid support by binding to avidin and used as ligands, or they may be hybridized to another RNA which acts as the ligand. The incorporat...

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Bibliographic Details
Published inMethods in enzymology Vol. 181; p. 97
Main Authors Ruby, S W, Goelz, S E, Hostomsky, Z, Abelson, J N
Format Journal Article
LanguageEnglish
Published United States 1990
Subjects
Actins - genetics
Base Sequence
Biotin
Chromatography, Affinity - methods
Exons
Indicators and Reagents
Molecular Sequence Data
Nucleic Acid Conformation
Ribonucleoproteins - isolation & purification
RNA - isolation & purification
RNA Precursors - isolation & purification
RNA Splicing
RNA, Fungal - isolation & purification
RNA, Messenger - genetics
Saccharomyces cerevisiae - genetics
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Summary:Small, reversibly biotinylated RNAs as described here are versatile ligands for affinity chromatography of RNA-binding components. These RNAs can be attached to a solid support by binding to avidin and used as ligands, or they may be hybridized to another RNA which acts as the ligand. The incorporation of a disulfide bond in the linker arm connecting biotin to the RNA makes it possible to dissociate the RNA from avidin under mild conditions. Our results regarding the binding and elution of the biotinylated RNA may be applied to other, reversibly biotinylated molecules.
ISSN:0076-6879
1557-7988
DOI:10.1016/0076-6879(90)81115-B