Immobilization of Cells on Polyurethane Foam

In this chapter, protocols and details for the immobilization of a model cell onto polyurethane foam carriers are provided in order to facilitate the use of such systems in laboratory or industrial reactors. Polyurethane foam has recently acquired great relevance as a carrier for its good mechanical...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2100; p. 407
Main Authors de Ory, Ignacio, Cabrera, Gema, Ramirez, Martin, Blandino, Ana
Format Journal Article
LanguageEnglish
Published United States 01.01.2020
Subjects
Adsorption
Cells, Immobilized
Mechanical Phenomena
Polyurethanes - chemistry
Porosity
Stirred tank
Adsorption
Packed column
Polyurethane foam
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Summary:In this chapter, protocols and details for the immobilization of a model cell onto polyurethane foam carriers are provided in order to facilitate the use of such systems in laboratory or industrial reactors. Polyurethane foam has recently acquired great relevance as a carrier for its good mechanical properties, high porosity, and large adsorption surface. In addition, it has a very low commercial cost. Two different immobilization protocols have been described, differing in the flow regime or the possibilities for the reactor: immobilization in a stirred tank reactor working in a discontinuous regime (by cycles) and immobilization in a packed column working in continuous operation mode. Protocols for carrier sterilization, analytical methodology, and immobilization are described.
ISSN:1940-6029
DOI:10.1007/978-1-0716-0215-7_27