Ganonorsterone A, a norsteroid from the medicinal fungus Ganoderma lingzhi

• Published in: Journal of asian natural products research Vol. 26; no. 8; pp. 1001 - 1008
• Main Authors: Du, Jia-Hui, Zhao, Xuan, Zhang, Fei, Wang, Yan, Du, Kun, Ding, Si-Yi, Feng, Wei-Sheng, Zhao, Zhen-Zhu
• Format: Journal Article
• Language: English
• Published: ABINGDON Taylor & Francis 02.08.2024; Taylor & Francis Ltd
• Subjects: Absolute configuration; Animals; Bioassays; Chemistry; Chemistry, Applied; Chemistry, Medicinal; Circular dichroism; Dichroism; Fruit bodies; Fruiting Bodies, Fungal - chemistry; Fungi; Ganoderma - chemistry; Ganoderma lingzhi; Life Sciences & Biomedicine; Macrophages; Macrophages - drug effects; Mice; Molecular Structure; Mushrooms; Nitric Oxide - antagonists & inhibitors; Nitric Oxide - biosynthesis; NMR; NO-production inhibition; norsteroid; Nuclear magnetic resonance; Pharmacology & Pharmacy; Physical Sciences; Plant Sciences; Quantum chemistry; quantum-chemical calculations; RAW 264.7 Cells; Science & Technology; quantum-chemical calculations; Ganoderma lingzhi; NO-production inhibition; STEROIDS; norsteroid
• ISSN: 1028-6020; 1477-2213; 1477-2213
• DOI: 10.1080/10286020.2024.2340691

Phytochemical investigation on the fruiting bodies of the medicinal fungus Ganoderma lingzhi led to the isolation of a new norsteroid, namely ganonorsterone A (1), together with one known steroid, cyathisterol (2). The structure and absolute configuration of compound 1 were assigned by extensive analysis of MS, NMR data, and quantum-chemical calculations including electronic circular dichroism (ECD) and calculated 13 C NMR-DP4+ analysis. Bioassay results showed that compound 1 displayed moderate inhibition on NO production in RAW 264.7 macrophages.