Repair of base-base mismatches in simian and human cells

Mismatched heteroduplexes arise as intermediates of several dissimilar genetic processes. The outcome of these genetic events will therefore be influenced by the efficiency and specificity of mismatch repair. We have studied the correction of base-base mispairs in simian and human fibroblasts by tra...

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Bibliographic Details
Published inGenome Vol. 31; no. 2; p. 578
Main Authors Brown, T C, Jiricny, J
Format Journal Article
LanguageEnglish
Published Canada 01.01.1989
Subjects
Animals
Base Composition
Base Sequence
Bloom Syndrome - genetics
Bloom Syndrome - physiopathology
Cells, Cultured
Cercopithecus aethiops
DNA Repair
DNA, Viral - genetics
Gene Conversion
Genetic Techniques
Humans
Molecular Sequence Data
Simian virus 40 - genetics
Transfection
Xeroderma Pigmentosum - genetics
Xeroderma Pigmentosum - physiopathology
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Summary:Mismatched heteroduplexes arise as intermediates of several dissimilar genetic processes. The outcome of these genetic events will therefore be influenced by the efficiency and specificity of mismatch repair. We have studied the correction of base-base mispairs in simian and human fibroblasts by transfecting the cells with derivatives of SV40 DNA, each harboring a single mispair in a defined orientation. Analysis of plaques revealed that correction efficiencies for homomispairs followed the pattern G.G greater than C.C greater than or equal to A.A greater than T.T. Repair bias was influenced by flanking sequences. Correction efficiences for heteromispairs followed the pattern of G.T greater than A.C greater than C.T greater than A.G and repair favored the retention of G + C by a substantial margin. This repair specificity could lead to a gene conversion bias favoring the accumulation of G + C in sequences subject to high levels of recombination or unequal exchange.
ISSN:0831-2796
DOI:10.1139/g89-107