DNA of Chinese hamster V79 cells newly synthesized after ultraviolet irradiation contains alkali-labile sites

Pulse-labeled daughter DNA of UV-irradiated Chinese hamster V79 cells was denatured in alkaline or neutral conditions and analysed by sucrose gradient centrifugation. A comparison of the sedimentation profiles of DNA treated in alkaline or neutral conditions has shown that in UV-irradiated cells som...

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Bibliographic Details
Published inMolecular & general genetics Vol. 198; no. 2; p. 183
Main Authors Pirsel, M, Sedliaková, M
Format Journal Article
LanguageEnglish
Published Germany 01.01.1984
Subjects
Alkalies
Animals
Cells, Cultured
Cricetinae
Cricetulus
DNA - biosynthesis
DNA - radiation effects
DNA Repair - radiation effects
Nucleic Acid Denaturation
Ultraviolet Rays
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Summary:Pulse-labeled daughter DNA of UV-irradiated Chinese hamster V79 cells was denatured in alkaline or neutral conditions and analysed by sucrose gradient centrifugation. A comparison of the sedimentation profiles of DNA treated in alkaline or neutral conditions has shown that in UV-irradiated cells some alkali-labile sites are produced during replication of damaged templates.
ISSN:0026-8925
DOI:10.1007/BF00328722