Polyamines enhance Agrobacterium tumefaciens vir gene induction and T-DNA transfer
We report here the enhancement of acetosyringone-mediated virulence gene induction in Agrobacterium tumefaciens by polyamines. The diamine putrescine and triamine spermidine enhanced vir gene induction when agrobacterial cells were treated prior to acetosyringone addition. An increase in polyamine l...
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Published in | Plant science (Limerick) Vol. 168; no. 2; pp. 475 - 480 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Shannon
Elsevier Ireland Ltd
01.02.2005
Elsevier Science |
Subjects | |
Online Access | Get full text |
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Summary: | We report here the enhancement of acetosyringone-mediated virulence gene induction in
Agrobacterium tumefaciens by polyamines. The diamine putrescine and triamine spermidine enhanced
vir gene induction when agrobacterial cells were treated prior to acetosyringone addition. An increase in polyamine levels was observed in
Agroacterium following the polyamine treatments, which was positively correlated with the magnitude of
vir gene induction. Plant transformation experiments have shown that modulation of polyamine levels in
Agrobacterium results in the enhanced T-DNA transfer. These results suggest the involvement of polyamines in the processes that lead to
vir gene induction and T-DNA transfer. And these findings may be useful in achieving a high transformation frequency in those plant species, which show minimal
vir gene induction. |
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ISSN: | 0168-9452 1873-2259 |
DOI: | 10.1016/j.plantsci.2004.09.018 |