Identification of Postharvest Senescence Regulators Through Map-Based Cloning Using Detached Arabidopsis Inflorescences as a Model Tissue

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1744; p. 195
• Main Authors: Hunter, Donald A, Jibran, Rubina, Dijkwel, Paul, Chagné, David, Sullivan, Kerry, Kanojia, Aakansha, Crowhurst, Ross
• Format: Journal Article
• Language: English
• Published: United States 2018
• Subjects: Aging; Arabidopsis - physiology; Biomarkers; Chlorophyll - metabolism; Chromosome Mapping; Genomics; Organ Specificity; Phenotype; Plant Physiological Phenomena; Polymorphism, Single Nucleotide; HRM; Senescence; WGS; High-resolution melting; Whole genome sequencing; Postharvest; EMS; Ethyl methanesulfonate
• ISSN: 1940-6029
• DOI: 10.1007/978-1-4939-7672-0_17

Postharvest deterioration of fruits and vegetables can be accelerated by biological, environmental, and physiological stresses. Fully understanding tissue response to harvest will provide new opportunities for limiting postharvest losses during handling and storage. The model plant Arabidopsis thaliana (Arabidopsis) has many attributes that make it excellent for studying the underlying control of postharvest responses. It is also one of the best resourced plants with numerous web-based bioinformatic programs and large numbers of mutant collections. Here we introduce a novel assay system called AIDA (the Arabidopsis Inflorescence Degreening Assay) that we developed for understanding postharvest response of immature tissues. We also demonstrate how the high-throughput screening capability of AIDA can be used with mapping technologies (high-resolution melting [HRM] and needle in the k-stack [NIKS]) to identify regulators of postharvest senescence in ethyl methanesulfonate (EMS) mutagenized plant populations. Whether it is best to use HRM or NIKS or both technologies will depend on your laboratory facilities and computing capabilities.